Hepatic transcriptional profiling in mouse models of Non-Alcoholic Fatty Liver Disease (NAFLD)

  • 9:00 am (PDT)
Speakers: Teresa Cardoso Delgado, PhD, Liver Disease Lab, CIC bioGUNE

In this webinar, Teresa Cardoso-Delgado, PhD from CIC bioGUNE discusses the impact of different dietary mouse models of non-alcoholic fatty liver disease (NAFLD) that are based on diets enriched in high fat or deprived of choline and methionine. Hepatic transcriptional profiling with the nCounter® Metabolic Pathways Panel revealed decreased expression of pathways responsible for amino acid synthesis and arginine metabolism.  Pathways involved in fatty acid metabolism and mitochondrial respiration decreased as the dietary model of NAFLD progressed, and, in late stages of NAFLD progression, pathways associated with hypoxia, DNA damage repair, myc, glutamine metabolism, mTOR and glycolysis, among others, were overexpressed, characterized by both overwhelming inflammation and fibrosis. In addition, immune cell profiling using cell typing signatures embedded in the Metabolic Pathways Panel revealed an enrichment of neutrophils, macrophages, dendritic cells, and CD45 cells for the most harmful dietary models, and these findings correlated with IHC. Overall, hepatic transcriptional profiling in animal models of dietary-induced NAFLD has been proven to be an important tool to assess disease progression and can be potentially used to evaluate therapeutics in preclinical studies.

For Research Use Only.  Not for use in Diagnostic Procedures.