Implementation of Fully Automated BOND RX Protocol for NanoString’s GeoMx Assay
NanoString’s GeoMx DSP allows for the characterization of spatial distribution and abundance of proteins and mRNAs with morphological context. This extends the ability for enhanced translational research in many areas, including immunology, oncology, and neuropathology with downstream analysis of analyte abundance by either nCounter or Next Generation Sequencing. The initial workflow with a semi-automated run on the BOND RX fully automated research stainer from Leica Biosystems was resource limiting due to heavy bench time for the RNA analysis. However, the development of a fully automated run on the BOND RX fully automated research stainer from Leica Biosystems for the RNA workflow allows a 4-fold increase in sample processing. This presentation will demonstrate the effectiveness of the new protocol and data generated by the two protocols compared across HNSCC samples.
Carlee Hemphill, MS
LabCorp Drug Development
Carlee Hemphill, MS, is a Lead Scientist I at the Covance CDx laboratory in RTP, NC with a background in histology, anatomical pathology, and bioinformatics. Her current role is the development of IHC or multiplex immunoflorescence assays, or computational pathology algorithms, for clients interested in a complementary or companion diagnostic testing assay for their immuo-oncology pharmaceutical drug development pipeline.
For Research Use Only. Not for Use in Diagnostic Procedures.