Next Generation Molecular Transplant Pathology: Early Experiences with the NanoString nCounter® Platform in Transplantation

  • 9:00 am (PDT)
Speakers: Dr. Benjamin A. Adam, MD, FRCPC, Assistant Professor of Pathology, University of Alberta

By allowing for reliable gene expression analysis of formalin-fixed paraffin-embedded (FFPE) tissue, the NanoString® nCounter® platform represents a potential game-changer for the field of molecular transplant pathology. This webinar will highlight some early experiences with this technology for applications related to transplantation, including: 1) technical validation of the nCounter system in archival FFPE human renal allograft biopsies; 2) confirmation of microarray-derived human molecular phenotypes in a nonhuman primate model for kidney transplantation using archival FFPE samples up to 20 years old; 3) use of an 800-gene panel to assess the molecular differences and similarities between renal allograft rejection and native kidney polyomavirus nephropathy; and 4) discovery of a molecular injury-repair response in ex vivo perfused porcine lung and heart transplants. Future opportunities will also be discussed, including: 1) the ability to perform spatially-resolved transcriptomics using the NanoString GeoMx™ Digital Spatial Profiling system; and 2) participation in international collaborative research initiatives facilitated by the Banff Foundation for Allograft Pathology, leveraging the new nCounter Human Organ Transplant Panel.

For Research Use Only.  Not intended for use in Diagnostic Procedures.