Spatially profiling acute T cell-mediated rejection in a kidney allograft
Transcriptional analysis of tissue graft biopsies’ represents a promising approach for studying previously unknown mechanisms in T cell-mediated–rejection (TCMR), also known as acute cellular rejection. However, bulk RNA sequencing of graft biopsies does not entirely capture the focal nature of acute rejection. A whole-transcriptome atlas approach with the GeoMX Digital Spatial Profiling platform was used to study five tubular and three glomerular regions of interest in the kidney graft biopsy from a patient with a chronic-active TCMR episode and in analogous areas from two different normal kidney control biopsies.
Hear about the power of Spatially profiling functional units of organ structures in tissue biopsies. Understand how previously unknown mechanisms for kidney graft rejection could be deciphered by studying once unappreciated variability across different tissue areas during TCMR, related to the graft’s response to alloimmune attack rather than to the immune cells.
Miguel Fribourg-Casajuana, PhD
Assistant Professor, Department of Medicine
Miguel Fribourg-Casajuana, Ph.D., is an engineer and immunologist. His lab focuses on multidisciplinary approaches that combine theoretical and experimental techniques to study cellular signaling in the context of organ transplant immunology to guide clinical intervention. Dr. Fribourg-Casajuana earned a Ph.D. in Signal Processing from the Polytechnical University in Madrid (UPM) and a Ph.D. in biomedical sciences, studying cell-signaling at an electrophysiology lab in Icahn School of Medicine at Mount Sinai in New York. He did five years of postdoctoral training in systems biology in the field of immunology, where he honed his skills in experimental techniques and computational analyses in functional genomics and studied the effects of type I interferons in early viral infections. Current research interests in the lab include the study of the role of interferon-beta in transplants and the development of new biosensors to monitor regulatory T cell (Treg) activity and function.