NanoString is pioneering the field of 3D Biology to accelerate the rate of research and maximize the amount of information that can be generated from a single sample. 3D Biology™ Technology is the ability to analyze combinations of RNA and protein simultaneously in a single experiment. Based on the NanoString digital barcode technology and designed for flexibility, the nCounter Vantage 3D Portfolio of assays provides a deeper view of cancer and immune biology and can be mixed and matched to answer a wide variety of biological questions.
How It Works
Vantage 3D Workflow
Vantage 3D Portfolio
Vantage 3D Workflow
The nCounter Vantage 3D portfolio is designed for flexibility. Each panel can also be run as a stand-alone assay so you can generate the data you need. Select the right targeted panels for each analyte type, and detect and analyze them all at once with the nCounter Analysis System and nSolver™ software.
Vantage 3D Portfolio
With the Vantage 3D portfolio, you can mix and match content based on your research needs with multi-analyte assays for Immuno-Oncology and Solid and Heme Tumor Profiling.
Panel Selection Tool
Find the gene expression panel for your research with easy to use panel pro
This panel focuses on the acquired immune system cells that help to identify and develop memory to invading pathogens. The panel will aid in examining the process by which the adaptive immune response is initiated, including markers of T-cell and B-cell activation, subpopulations, and key signaling molecules. Some gene content also relates to antigen presentation and checkpoint genes.
The innate immune system includes cell types that mount the early response to invading pathogens and are required to create a robust adaptive immune response. This arm of the immune system involves natural killer cells, mast cells, eosinophils, basophils, macrophages, neutrophils, and dendritic cells. Innate immune genes involved in host response, bacterial sensing, inflammation, Toll-like receptor signaling, and related cytokines are included in this panel.
Altered tumor metabolism is widely recognized as the “Forgotten Hallmark” that allows for the tumor cell to thrive and proliferate. Cancer metabolism has many facets, from aerobic glycolysis and glucose uptake to energy consumption. This panel includes genes from these various areas.
The JAK/STAT pathway relays cellular cues into an intracellular transcription response. The pathway is crucial to immune response, as many of its receptors bind to cytokines. This panel includes cytokines, cytokine receptors, and key targets in the JAK/STAT signaling pathway, as well as related targets from the PI3K/AKT pathway and mTOR pathway
In addition to the 109 cell typing genes from the PanCancer Immune Profiling Panel, the cellular profiling panel includes an additional 40 genes to give a more in-depth view of immune cell populations. Also included are markers for cells that interact with the immune system including endothelial, smooth muscle, fibroblast, and stromal cells.
The Wnt signaling pathway regulates the developmental processes of cell fate and plays a role in tumorigenesis and progression. Wnt signaling comprises three pathways: the canonical pathway and two non-canonical pathways, planar cell polarity (PCP) and a calcium ion-dependent pathway. The canonical pathway is dependent on beta-catenin and regulates the cell cycle, cell growth, and proliferation. This panel includes the Wnt signaling ligands and receptors as well as other auxiliary signaling molecules for regulation, proteolysis, cell migration, cell cycle, adhesion, and EMT/metastasis.
Exposure to specific agents (such as reactive oxygen species, methylating agents, etc.) and even normal physiological processes all damage DNA. Cells must repair DNA damage to prevent mutations from propagating into cancer and accumulating. This panel includes the genes involved in major DNA damage repair pathways including base excision repair, nucleotide excision repair, mismatch repair, translesion synthesis, and other repair processes. Additional genes from apoptosis and cell cycle pathways are included.
PRODUCT DESCRIPTION
CATALOG NUMBER
UNIT SIZE
Gene Expression CodeSet profiling (192 genes) 180 DNA Damage and Repair-related human genes + 12 internal reference controls. CodeSet Only.
The MAPK and PI3K pathways control key cellular processes in development, homeostasis, and disease. A variety of extracellular signals converge on these pathways, leading to the expression of genes controlling multiple cellular and tissue-level functions. Seminal publications focused on MAPK and PI3K research and signature development were collated and scored to develop a curated gene set measuring transcriptional activity of these key pathways. This panel represents the diverse set of biological functions that the MAPK and PI3K pathways modulate as crucial mediators of biological activity.
Despite diversity in cell of origin and other clinical features, hematologic malignancies share a core set of pathways and processes that drive tumorigenesis, such as MAPK, MYC signaling, NF-κB, PI3K-AKT, and B cell and T cell receptor signaling. Additionally, dysregulation of cytokines, apoptosis, the DNA damage response, and epigenetic factors are frequently observed. Seminal publications focused on hematologic oncology research and signature development were collated and scored to develop a curated gene set that measures these conserved and crucial pathways underlying hematologic malignancies.
Vantage 3D Lung Fusion Panel includes 63 probes: 35 for specific fusion detection, 24 for positional gene expression imbalance detection, and 4 internal reference genes.
PRODUCT
DESCRIPTION
CATALOG NUMBER
UNIT SIZE
nCounter® Vantage 3D™ Lung Gene Fusion Panel
Includes 63 probes: 35 for specific fusion detection, 24 for positional gene expression imbalance detection, and 4 internal reference genes. Master Kit reagents sold separately.
Track protein expression of key cell surface receptors, lineage markers, and regulatory molecules to monitor shifts in cell type abundance and activation status. The Immune Cell Profiling panel enables precise, digital detection of the most important cell surface targets in your immuno-oncology research.
PRODUCT
DESCRIPTION
CATALOG NUMBER
UNIT SIZE
nCounter® Vantage 3D™ Protein Immune Cell Profiling Panel for cell suspensions (D) + Universal Cell Capture Kit
Contains Antibody Mix for 30 Extracellular immunology protein targets. Protein TagSet (D) (ERCC) for protein target detection. Universal Cell Capture Kit—Cell Surface Compatible, which contains universal cell capture beads, Buffer W, and Buffer LH. To be used as a stand-alone protein assay. Compatible with cell suspensions.
VPODC-B2M-HIPS-12
12 reactions
nCounter® Vantage 3D™ Protein Immune Cell Profiling Panel for cell suspensions (R) + Universal Cell Capture Kit
Contains Antibody Mix for 30 Extracellular immunology protein targets. Protein TagSet (R) (no ERCC) for protein target detection. Universal Cell Capture Kit—Cell Surface Compatible, which contains universal cell capture beads, buffer W, and buffer LH. To be used with custom CodeSets, PanCancer RNA Panels, and nCounter® Vantage 3D RNA Panels. Compatible with cell suspensions.
Perform multiplex protein analysis covering key solid tumor signaling pathways, including MAPK, PI3K, and EGFR, all with a single antigen retrieval step. Carefully selected content designed with your solid tumor research in mind provides comprehensive pathway profiling from minimal FFPE sample.
PRODUCT
DESCRIPTION
CATALOG NUMBER
UNIT SIZE
Vantage 3D Protein Solid Tumor Panel for FFPE (D) + FFPE Sample Prep Kit
Contains: Antibody Mix for 26 solid tumor signaling protein targets. Protein TagSet (D) (ERCC) for protein target detection. Protein FFPE Sample Prep Kit, which contains Buffer W and Buffer T. To be used as a stand-alone protein assay. Compatible with FFPE samples.
VPODC-SPKP-HSTF-12
12 reactions
nCounter Vantage 3D Protein Solid Tumor Panel for FFPE (R) + FFPE Sample Prep Kit
Contains: Antibody Mix for 26 solid tumor signaling protein targets. Protein TagSet (R) (no ERCC) for protein target detection. Protein FFPE Sample Prep Kit, which contains Buffer W and Buffer T. To be used with custom CodeSets, PanCancer RNA Panels, and nCounter Vantage 3D RNA Panels. Compatible with FFPE samples.
VPRXC-SPKP-HSTF-12
12 reactions
Perform multiplex analysis of phospho- and total proteins associated with key hematology-oncology signaling pathways, including PI3K, BCR, and TCR signaling, all with a single antigen retrieval step. The panel content is designed to provide comprehensive pathway profiling from minimal FFPE sample.
Product
Description
Catalog Number
Unit Size
Vantage 3D Protein Heme Panel (D) for FFPE
Contains: Antibody Mix and Protein TagSet (D) reagent (ERCC) for protein target detection. Buffer W and Buffer T for protein sample preparation. To be used as a stand-alone protein assay or with nCounter Vantage 3D DNA assays. Compatible with FFPE samples.
VPODC-HHMF-12
12 reactions
Vantage 3D Protein Heme Panel (R) for FFPE
Contains: Antibody Mix and Protein TagSet (R) reagent (no ERCC) for protein target detection. Buffer W and Buffer T for protein sample preparation. To be used with custom CodeSets, PanCancer RNA Panels, and nCounter Vantage 3D RNA Panels. Compatible with FFPE samples.
VPRXC-HHMF-12
12 reactions
Panel Compatibility and Customization
Each panel can be run as a stand-alone assay with customization available. Up to 24 user- defined genes can be added to any Vantage 3D RNA panel, including fusion panels. Up to five custom protein targets can be added to any Vantage 3D Protein panel using the Protein Barcoding Service.
Unsure if you can perform your desired experiment in a single lane? The table below provides an overview of analyte compatibility:
Digital Spatial Profiling Reveals Functional Shift of Enterochromaffin Cell in Patients With Ulcerative Colitis.
As a major component of the enteroendocrine system, enterochromaffin (EC) cells play a key role in ulcerative colitis (UC). However, the scarcity of EC cells has limited the investigation of their function.
PSMA-targeting TGFbeta-insensitive armored CAR T cells in metastatic castration-resistant prostate cancer: a phase 1 trial.
Chimeric antigen receptor (CAR) T cells have demonstrated promising efficacy, particularly in hematologic malignancies. One challenge regarding CAR T cells in solid tumors is the immunosuppressive tumor microenvironment (TME), characterized by high levels of multiple inhibitory factors, including transforming growth factor (TGF)-beta.
Immunostimulatory cancer-associated fibroblast subpopulations can predict immunotherapy response in head and neck cancer.
Purpose: Cancer-associated fibroblasts (CAF) have been implicated as potential mediators of checkpoint immunotherapy response. However, the extensive heterogeneity of these cells has precluded rigorous understanding of their immunoregulatory role in the tumor microenvironment.
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