1 Dataset Introduction

1.1 CosMx SMI Instrument Background

CosMx SMI is currently the only spatial platform enabling quantification of 1000+ targets at subcellular resolution for maximum biological insight. The CosMx SMI in situ chemistry is designed to enable high-plex and high-resolution with probes and fluorophores designed to reduce optical crowding limitations, allowing multiplexing probes to 1000 unique transcript species and beyond. In addition to high-plex, CosMx SMI also achieves high resolution. Target localization accuracy of ∼50 nm in the XY plane allows for targeting of <1kb transcripts with no loss in performance.