Dynamically Choose Housekeepers
Uses the geNorm algorithm (Vandesompele et al., Genome Biology, 2002) to identify the most stably expressed genes up to the number specified from among the annotated housekeepers and normalizes the data using these genes. This option is recommended for use with raw data. Selection of this option with normalized data will override normalization performed in the experiment wizard.
Run QC and Descriptive Analyses
Selection of this option generates descriptive plots, including heatmaps and principal component analysis of the whole dataset and of smaller gene sets, and summaries of experimental variables' distribution and association with gene expression. This option is recommended for all analyses.
Column Defining Gene Sets
Choose annotation information for all gene set analyses. By default, gene sets will be annotated using information defined in the Immune Response Category column.