Drive Research Efficiency with Fit for Purpose Gene Expression Solutions
Optimize Your Workflow with nCounter® Analysis
As scientific research continues to grow in complexity, it becomes increasingly crucial to develop an ecosystem of specialized fit for purpose gene expression solutions, each designed to address specific challenges. These fit for purpose gene expression solutions will enhance the overall efficiency and accuracy of the research process.
Establishing an ecosystem with fit for purpose gene expression solutions enables:
- Enhanced experimental results
- Increased productivity
- Better utilization of resources
- Innovation cultivation
- Enhanced adaptability and flexibility
- Time and money savings
The Benefits of Integrating nCounter Into Your Lab Ecosystem
Many labs are equipped with gene expression technologies and instrumentation that are actually highly complementary of one another. Common lab equipment includes PCR and NGS instruments. Having access to nCounter® direct detection analysis also offers unique benefits that complement the ecosystem. The benefits of utilizing all these fit for purpose gene expression solutions includes expedited results and enhanced research efficiency.
Key Applications to Maximize Lab Efficiencies
There are many applications that would benefit from utilizing fit for purpose gene expression solutions. Integrating the nCounter Analysis System into workflows that include translational research and pathway-based applications is a great start to enhancing efficiencies in your lab.
Spotlight on Success
Biomarker Development
“NanoString nCounter® PanCancer IO360™ can generate a similar immune profile to that generated by the WTS platform in advanced melanoma patients…it is also a reliable and practical approach to analyse the variations of immune signatures between response status and conduct cross-platform analysis.”
Mao et. al.
Reproduced from Mao et al. J Transl Med 2023; 21:257
Biomarker Development
“It is possible to conduct multi-omic studies in a complex multi-institutional setting for investigations involving minute quantities of FFPE tumors, as in studies of early-stage melanoma.”
Orlow et. al.
Reproduced from Orlow et al PLoS ONE 2023: 18(4)
Immune Response
“The case of MPV illustrates the potential superiority of NanoString over RNA-Seq for certain viral infections where early and accurate detection of a small number of genes is critical. NanoString’s methodology allows for a more direct and quantifiable approach…”
Rezapour et. al.
Reproduced from Rezapour et al Front. Genet. 2024: 15:1327984
Multi-Omics & Spatial Biology
“This is the first comprehensive study that connects the immune landscape of gene expression profiles and protein spatial infiltration with the survivorship of PDAC patients.”
Aziz et. al.
Reproduced from Aziz et al 2023: Front. Immunol. 13:995715
Multi-Omics & Spatial Biology
“Gene expression for all GeoMx regions correlated well with the corresponding matched [IO360] bulk sample…”
“This study illustrates the potential to leverage ST performed on the GeoMx DSP to characterize tumor heterogeneity, and identify novel biomarkers associated with clinically relevant subtypes of colorectal cancer.”
Wood et. al.
Reproduced from Wood et al 2023: Cancer Res 83 (8): 1329–1344
nCounter Analysis System Strengths
Actionable Answers
nCounter’s thematic gene expression panels were developed using amassed sequencing knowledge and input from industry experts. The panels are designed to deliver actionable biology versus just more data.
Simple and Efficient Workflow
The nCounter workflow requires much less hands-on time than other tools. The nCounter workflow features walk-away automation and less than 15 minutes of hands-on time. Most protocols require only 4 pipetting steps. Automation also makes it easy to scale for many samples.
Reduced Variability
The nCounter chemistry requires a limited number of steps, which reduces potential sources of variability.
Speed
Get results in less than 24 hours with the nCounter Analysis System.
PCR
Same day results
Weeks of Optimization
NGS
Days to weeks for data
Weeks of analysis with bioinformatics
nCounter
Results within 24-hours
Easy Data Analysis
When accessible data is essential for your research the nCounter Analysis System is your go-to solution. With a simple 4-step analysis protocol actionable answers are right at your fingertips. Generate highly tailored exports, basic statistical outputs, and publication-quality figures quickly and easily with no incremental cost or need for bioinformatics expertise.
- Base Calls/Raw Data QC
- Filtering & Trimming
- Read Alignment
- Reference Transcriptome alignment
- Post Alignment Processing
- Read Counting
- Measure Transcript Levels
- Normalize
- ID Differentially Expressed Genes
- Annotate Genes
- Visualize Data
Publications
Cross-platform comparison of immune signatures in immunotherapy-treated patients with advanced melanoma using a rank-based scoring approach
Background: Gene expression profiling is increasingly being utilised as a diagnostic, prognostic and predictive tool for managing cancer patients. Single-sample scoring approach has been developed to alleviate instability of signature scores due to variations from sample composition.
Comparison of RT-qPCR and Nanostring in the measurement of blood interferon response for the diagnosis of type I interferonopathies
A comparative analysis of RNA-Seq and NanoString technologies in deciphering viral infection response in upper airway lung organoids
In this study, we delved into the comparative analysis of gene expression data across RNA-Seq and NanoString platforms. While RNA-Seq covered 19,671 genes and NanoString targeted 773 genes associated with immune responses to viruses, our primary focus was on the 754 genes found in both platforms.
Comparison of Metagenomic Sequencing and the NanoString nCounter Analysis System for the Characterization of Bacterial and Viral Communities in Vaginal Samples
DNA sequencing assays have been used to characterize the vaginal microbiome and to identify associations with clinical outcomes. The purpose of this study was to evaluate the utility of the NanoString nCounter platform, a more efficient assay compared to sequencing, for the characterization of vaginal microbial communities.
Application of the NanoString nCounter System as an Alternative Method to Investigate Molecular Mechanisms Involved in Host Plant Responses to Plasmodiophora brassicae
Clubroot, caused by the soilborne pathogen Plasmodiophora brassicae, is an important disease of canola (Brassica napus) and other crucifers. The recent application of RNA sequencing (RNA-seq) technologies to study P.