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The Fine Balance of Immunology

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The immune system often plays a role in the onset and progression of different disorders such as autoimmunity, fibrosis, and cancer. Understanding which cell types, cytokines, pathways, and transcription factors are involved in the balance between immune activation and suppression can lead to better treatments for chronic inflammation and disease. In addition to traditional bulk expression and proteomic analysis, spatial profiling of the location of immune cells in tissue can yield a better understanding of disease pathogenesis, uncovering differences within individuals as well as novel biomarkers for stratification and treatment.

Challenges

We know it’s a challenge as an immunologist to piece together what underlies healthy versus exhausted or abnormal immune system function. Projects often involve many different research techniques, cell types, and biomolecules and you may be working with a variety of sample types.  Even more of a challenge is understanding how different immune cells and biomolecules function and communicate in situ in the tissue in response to disease. 

How much could you advance your understanding of the immune system if you had access to a multiplexed technology platform for transcriptomic and proteomic analysis of multiple sample types such as Formalin-Fixed, Paraffin-Embedded (FFPE) tissue sections, fresh frozen tissue, cell lysates, PBMCs and whole blood? Make an impact on human health faster with streamlined bulk and spatial analysis of RNA, protein, and immune cell types using the combined power of the nCounter® Analysis System and the GeoMx® Digital Spatial Profiler.


Publications

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Idelalisib inhibits experimental proliferative vitroretinopathy.

Proliferative vitreoretinopathy (PVR) is a fibrotic eye disease that develops after rhegmatogenous retinal detachment surgery and open-globe traumatic injury. Idelalisib is a specific inhibitor of phosphoinositide 3-kinase (PI3K) δ.

Comparative spatial transcriptomic and single-cell analyses of human nail units and hair follicles demonstrate transcriptional similarities between the onychodermis and follicular dermal papilla.

The nail unit and hair follicle are both hard keratin-producing organs that share various biological features. Here we used a Digital Spatial Profiling (DSP) and single cell RNA sequencing (scRNA-seq) to define a spatially resolved expression profile of the human nail unit and hair follicle.

Prevalence and Mechanisms of Mucus Accumulation in COVID-19 Lung Disease.

Rationale: The incidence and sites of mucus accumulation, and molecular regulation of mucin gene expression, in COVID-19 lung disease have not been reported. Objectives: Characterize incidence of mucus accumulation and the mechanisms mediating mucin hypersecretion in COVID-19 lung disease.

Related Resources

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Product Bulletin Autoimmune Profiling Panel – Product Bulletin
Updating immune cell deconvolution for the spatial genomics era
Webinar Defining the function of genes associated with chronic inflammatory diseases: The nCounter® Autoimmune Discovery Consortium Panel
Blog The Chronic(als) of Autoimmunity
Blog Solid Organ Transplantation and the Immunology of Rejection. Q&A with GeoMx® Digital Spatial Profiler Grant Winner Dr. Fadi Issa