The Fine Balance of Immunology
The immune system often plays a role in the onset and progression of different disorders such as autoimmunity, fibrosis, and cancer. Understanding which cell types, cytokines, pathways, and transcription factors are involved in the balance between immune activation and suppression can lead to better treatments for chronic inflammation and disease. In addition to traditional bulk expression and proteomic analysis, spatial profiling of the location of immune cells in tissue can yield a better understanding of disease pathogenesis, uncovering differences within individuals as well as novel biomarkers for stratification and treatment.
Challenges
We know it’s a challenge as an immunologist to piece together what underlies healthy versus exhausted or abnormal immune system function. Projects often involve many different research techniques, cell types, and biomolecules and you may be working with a variety of sample types. Even more of a challenge is understanding how different immune cells and biomolecules function and communicate in situ in the tissue in response to disease.
How much could you advance your understanding of the immune system if you had access to a multiplexed technology platform for transcriptomic and proteomic analysis of multiple sample types such as Formalin-Fixed, Paraffin-Embedded (FFPE) tissue sections, fresh frozen tissue, cell lysates, PBMCs and whole blood? Make an impact on human health faster with streamlined bulk and spatial analysis of RNA, protein, and immune cell types using the combined power of the nCounter® Analysis System and the GeoMx® Digital Spatial Profiler.
Featured Solutions
Choose from curated, multiplexed nCounter Gene Expression Panels, nCounter Vantage 3D™ Protein Assays, GeoMx DSP RNA Assays, and/or GeoMx Protein Assays to build your next experiment and discover answers to your immunology questions. Take advantage of embedded immune cell typing signatures in nCounter expression panels to quantify the relative abundance of 14 different immune cell types or run an nCounter panel downstream of FACs to profile specific immune cell populations.
Publications
The Technical Feasibility of Digital Spatial Profiling in Immune/Inflammation Study of Thrombosis
Background: A comprehensive study of the distribution and role of immune/inflammatory cells in thrombosis is still lacking because traditional pathology techniques cannot accomplish the analysis of numerous protein and genetic data simultaneously. We aimed to evaluate the feasibility of digital spatial profiling (DSP) to study immune/inflammation reaction in thrombosis progression.
Morphologic and molecular analysis of liver injury after SARS-CoV-2 vaccination reveals distinct characteristics
Background and Aims: Liver injury after COVID-19 vaccination is very rare and shows clinical and histomorphological similarities with autoimmune hepatitis (AIH). Little is known about the pathophysiology of COVID-19 vaccine-induced liver injury (VILI) and its relationship to AIH.
Pan-cancer T cell atlas links a cellular stress response state to immunotherapy resistance
Tumor-infiltrating T cells offer a promising avenue for cancer treatment, yet their states remain to be fully characterized. Here we present a single-cell atlas of T cells from 308,048 transcriptomes across 16 cancer types, uncovering previously undescribed T cell states and heterogeneous subpopulations of follicular helper, regulatory and proliferative T cells.
Related Resources
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