The Fine Balance of Immunology

The immune system often plays a role in the onset and progression of different disorders such as autoimmunity, fibrosis, and cancer. Understanding which cell types, cytokines, pathways, and transcription factors are involved in the balance between immune activation and suppression can lead to better treatments for chronic inflammation and disease. In addition to traditional bulk expression and proteomic analysis, spatial profiling of the location of immune cells in tissue can yield a better understanding of disease pathogenesis, uncovering differences within individuals as well as novel biomarkers for stratification and treatment.

Challenges

We know it’s a challenge as an immunologist to piece together what underlies healthy versus exhausted or abnormal immune system function. Projects often involve many different research techniques, cell types, and biomolecules and you may be working with a variety of sample types.  Even more of a challenge is understanding how different immune cells and biomolecules function and communicate in situ in the tissue in response to disease. 

How much could you advance your understanding of the immune system if you had access to a multiplexed technology platform for transcriptomic and proteomic analysis of multiple sample types such as Formalin-Fixed, Paraffin-Embedded (FFPE) tissue sections, fresh frozen tissue, cell lysates, PBMCs and whole blood? Make an impact on human health faster with streamlined bulk and spatial analysis of RNA, protein, and immune cell types using the combined power of the nCounter® Analysis System and the GeoMx® Digital Spatial Profiler.

Publications

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Multiplexed Digital Spatial Protein Profiling Reveals Distinct Phenotypes of Mononuclear Phagocytes in Livers with Advanced Fibrosis.

Background and Aims: Intrahepatic mononuclear phagocytes (MPs) are critical for the initiation and progression of liver fibrosis. In this study, using multiplexed digital spatial protein profiling, we aimed to derive a unique protein signature predicting advanced liver fibrosis.

Spatial Profiling of the Prostate Cancer Tumor Microenvironment Reveals Multiple Differences in Gene Expression and Correlation with Recurrence Risk.

Simple Summary: In prostate cancer (PCa), the tumor microenvironment plays a crucial role in both the development and progression of the disease. We used Digital Spatial Profiling multiplex technology to assess the expression of 58 protein and 1825 RNA transcripts in traditionally challenging samples (i.

Chronic stress induces colonic tertiary lymphoid organ formation and protection against secondary injury through IL-23/IL-22 signaling.

Psychological stress has been previously reported to worsen symptoms of inflammatory bowel disease (IBD). Similarly, intestinal tertiary lymphoid organs (TLOs) are associated with more severe inflammation.

Related Resources

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Product Bulletin Autoimmune Profiling Panel – Product Bulletin
Updating immune cell deconvolution for the spatial genomics era
Webinar Defining the function of genes associated with chronic inflammatory diseases: The nCounter® Autoimmune Discovery Consortium Panel
Blog Post Q&A with Dr. Salla Keskitalo: Challenges and Opportunities in Studying Rare and Ultra-rare Autoimmune Diseases
Blog Post Solid Organ Transplantation and the Immunology of Rejection. Q&A with GeoMx® Digital Spatial Profiler Grant Winner Dr. Fadi Issa
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