GeoMx® Protein Assays

Helping Your Research

High-plex Spatial Proteomic Analysis

GeoMx Protein Assays enable quantitative, spatial analysis of 96+ proteins from a single FFPE or Fresh Frozen slide, vastly expanding the number of markers you can profile from a single tissue section compared to traditional immunohistochemical methods. All assays are validated for use in multiplex so you can focus on the biology of interest without wasting time troubleshooting assay performance. 

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How it Works

The assay relies upon antibodies coupled to photocleavable oligonucleotide tags designed for readout with nCounter® (≤96-plex) or NGS (over 100-plex) platforms. After hybridization of antibodies to slide-mounted tissue sections, the oligonucleotide tags are released from discrete regions of the tissue via UV exposure. Released tags are counted in a standard nCounter assay or sequenced using NGS, and counts are mapped back to tissue location yielding a spatially resolved digital profile of protein abundance.

All GeoMx® Protein Assays undergo extensive validation to ensure high quality GeoMx DSP data. Read more about GeoMx Antibody Validation in this white paper.

Product Information

Spatial Proteogenomics
GeoMx Protein Assays for nCounter
GeoMx Protein Assays for NGS
Customize your GeoMx Protein Assays
Spatial Proteogenomics

Spatial Proteogenomics

Simplify your multiomics studies with the multimodal Spatial Proteogenomics workflow. This workflow enables co-detection of RNA and protein from the same tissue slide on GeoMx® DSP with NGS as the readout method. Harness the sensitivity of both GeoMx RNA Assays and GeoMx Protein Assays to capture a complete picture of your biology starting with transcription to translation with capabilities to measure protein activation and post-translational modifications.

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GeoMx Protein Assays for nCounter

Immuno-Oncology and Immunology

The Immune Cell Profiling Core contains 18 targets for broad cell profiling of human samples (17 targets for mouse) and includes necessary controls for all GeoMx DSP experiments. Up to 6 10-plex modules plus 10 custom targets can be added to select the content most relevant to your human and mouse research.

 

Neuroscience

The Neural Cell Profiling Core contains 20 targets designed for broad cell profiling of human samples (19 targets for mouse) and includes necessary controls for all GeoMx DSP experiments. Up to 6 10-plex modules plus 10 custom targets can be added to select the content most relevant to your human and mouse research.

GeoMx Protein Assays for NGS

Designed to profile 10’s-100’s of protein targets simultaneously with spatial resolution using NGS platforms and pipelines. Run the 4-plex GeoMx Protein Core for NGS, which includes necessary controls for GeoMx DSP experiments, with any selection of 10-plex modules.

Customize your GeoMx Protein Assays

As part of the overall Custom Protein Workflow, the GeoMx® Protein Barcoding Kit enables researchers to barcode antibodies of interest with a GeoMx DSP barcode in a single day with minimal hands-on time. Once antibodies have been barcoded, researchers can continue with their Custom Protein Workflow for any further appropriate functional testing. When custom antibodies are barcoded with the GeoMx Protein Barcoding kit, the addition of this content to GeoMx Protein Assays enables profiling up to 150 plex in a single experiment.

 

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Related Resources

See All Resources
Product Bulletin GeoMx Protein Barcoding Kits – Product Bulletin
Product Bulletin GeoMx IO & Immune Protein Assays for nCounter – Product Bulletin
Product Bulletin GeoMx Human Protein Assays for NGS – Product Bulletin
Product Bulletin GeoMx Neuro Protein Assays for nCounter – Product Bulletin
Product Bulletin GeoMx® Mouse Protein Assays-Product Bulletin

Publications

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Spatial transcriptomic characterization of COVID-19 pneumonitis identifies immune circuits related to tissue injury.

Severe lung damage resulting from COVID-19 involves complex interactions between diverse populations of immune and stromal cells. In this study, we used a spatial transcriptomics approach to delineate the cells, pathways, and genes present across the spectrum of histopathological damage in COVID-19-affected lung tissue.

Highly Multiplexed Spatially Resolved Proteomic and Transcriptional Profiling of the Glioblastoma Microenvironment Using Archived Formalin-Fixed Paraffin-Embedded Specimens.

Glioblastoma is a heterogeneous tumor for which effective treatment options are limited and often insufficient. Few studies have examined the intratumoral transcriptional and proteomic heterogeneity of the glioblastoma microenvironment to characterize the spatial distribution of potential molecular and cellular therapeutic immunooncology targets.

PFKFB3 overexpression in monocytes of patients with colon but not rectal cancer programs pro-tumor macrophages and is indicative for higher risk of tumor relapse.

Introduction: Circulating monocytes are main source for tumor-associated macrophages (TAMs) that control tumor growth, angiogenesis, metastasis and therapy resistance. We raised the questions how monocyte programming is affected by growing tumors localized in colon and rectal sections, and how treatment onsets affect monocyte programming in the circulation.

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