Support Documentation

User Manuals

GeoMx Digital Spatial Profiler

Find PDF Manuals & Quick Reference Guides in the NanoString University Document Library.

Gene Expression

• Gene Expression Overview MAN-10083
  • Preparing Nucleic Acid from FFPE Samples for Use with nCounter Assays MAN-10050
  • Preparing RNA and Lysates from Fresh Frozen Samples for Use with nCounter Assays MAN-10051
  • Low RNA Input Amplification Kit (Optional) MAN-10046
  • Gene Expression RNA CodeSet Hybridization Protocol MAN-10056
• Design
  • Expression CodeSet Design Manual MAN-C0002

Panel-Plus and CodeSet-Plus

• Gene Expression RNA CodeSet Hybridization Protocol MAN-10056

Elements™ Reagents

• Elements XT RUO User Manual (USA) MAN-10086 (Replaced LBL-C0266)
• Elements XT FLU User Manual (Ex-US) LBL-10241 Document Retired March 1, 2020

PlexSet™

• nCounter PlexSet Reagents User Manual MAN-10040
• All About PlexSet Data Analysis with nSolver™ Software MAN-10044
• nCounter PlexSet Technology Setup Quick Reference MAN-10079

miRNA

• miRNA Expression Assay User Manual MAN-C0009
• miRGE Assay Manual MAN-C0017

CNV

• CNV Hybridization Protocol MAN-10093

Gene Fusion

• Gene Fusion Panels Overview MAN-10085
  • Preparing Nucleic Acid from FFPE Samples for Use with nCounter Assays MAN-10050
  • Preparing RNA and Lysates from Fresh Frozen Samples for Use with nCounter Assays MAN-10051
  • Gene Fusion RNA TagSet Hybridization Protocol MAN-10058

3D Biology

• RNA Expression
  • Vantage 3D RNA Panels Overview MAN-10084
    • Preparing Nucleic Acid from FFPE Samples for Use with nCounter Assays MAN-10050
    • Preparing RNA and Lysates from Fresh Frozen Samples for Use with nCounter Assays MAN-10051
    • Low RNA Input Amplification Kit (Optional) MAN-10046
    • TagSet Hybridization Setup MAN-10057
• nCounter^® Vantage 3D™ RNA:Protein Immune Cell Profiling Panel
  • Overview MAN-10031
    • Cell Surface Protein Processing from Cell Suspensions MAN-10066
    • Low RNA Input Amplification Kit (Optional) MAN-10046
    • RNA:Protein Hybridization Setup MAN-10060
• nCounter^® Vantage 3D™ RNA:Protein Solid Tumor Panel for Lysate
  • Assay Overview MAN-10033
    • Sample Prep (RNA Overview) MAN-10051
    • Sample Prep (Protein ± RNA) MAN-10054
    • Low RNA Input Amplification Kit (Optional) MAN-10046
    • RNA:Protein Hybridization MAN-10060
• nCounter^® Vantage 3D™ RNA:Protein Solid Tumor Panel for FFPE
  • Assay Overview MAN-10035
    • Sample Prep (RNA) MAN-10050
    • Sample Prep (Protein) MAN-10053
    • Low RNA Input Amplification Kit (Optional) MAN-10046
    • RNA:Protein Hybridization MAN-10060
• nCounter^® Vantage 3D™ RNA:Protein Heme Assay for Lysate
  • Assay Overview MAN-10072
    • Sample Prep (Nucleic Acid) MAN-10051
    • Sample Prep (Protein ± RNA) MAN-10054
    • Low RNA Input Amplification Kit (Optional) MAN-10046
    • RNA:Protein Hybridization MAN-10065
• nCounter^® Vantage 3D™ RNA:Protein Heme Assay for FFPE
  • Assay Overview MAN-10073
    • Sample Prep (Nucleic Acid) MAN-10050
    • Sample Prep (Protein) MAN-10053
    • Low RNA Input Amplification Kit (Optional) MAN-10046
    • RNA:Protein Hybridization MAN-10065

Protein Expression

• nCounter^®Vantage 3D™Protein Solid Tumor Panel for Lysates
  • Assay Overview MAN-10034
    • Sample Prep MAN-10054
    • Protein Only Hybridization Setup MAN-10059
• nCounter^®Vantage 3D™Protein Solid Tumor Panel for FFPE
  • Assay Overview MAN-10036
    • Sample Prep MAN-10053
    • Protein Only Hybridization Setup MAN-10059
• Vantage 3D™ RNA:Protein Heme Assay for Lysate
  • Assay Overview MAN-10072
    • Sample Prep (Nucleic Acid) MAN-10051
    • Sample Prep (Protein ± RNA) MAN-10054
    • Low RNA Input Amplification Kit (Optional) MAN-10046
    • RNA:Protein Hybridization MAN-10065
• Vantage 3D™ RNA:Protein Heme Assay for FFPE
  • Assay Overview MAN-10073
    • Sample Prep (Nucleic Acid) MAN-10050
    • Sample Prep (Protein) MAN-10053
    • Low RNA Input Amplification Kit (Optional) MAN-10046
    • RNA:Protein Hybridization MAN-10065

PAM50

• PAM50 RUO Hybridization Protocol – Manual MAN-10125

Lymphoma Subtyping Test (LST)

• LST RUO User Protocol – Manual MAN-10142

Systems

• nCounter Sprint Profiler
  • nCounter SPRINT® Profiler User Manual MAN-10017
  • Loading Protocol for nCounter® SPRINT Cartridges
  • How to Send SPRINT Log Files
• nCounter Pro
  • nCounter Pro Analysis System User Manual SEV-00184
• nCounter MAX/FLEX
  • nCounter Analysis System User Manual (MAX/FLEX) MAN-C0035
  • nCounter MAX/FLEX System Quick Start Guide GLMX_ST0002
  • How to Send MAX/FLEX Log Files

nSolver Software and Analysis

• nSolver 4.0 Analysis Software User Manual MAN-C0019
• nCounter Advanced Analysis Plugin for nSolver Software User Manual MAN-10030
• nCounter Data Analysis Guide MAN-C0011
• nCounter Data Analysis Guidelines for Copy Number Variation (CNV) MAN-C0014
• nSolver Quick Start Guide

Tech Notes and White Papers

GeoMx® Digital Spatial Profiler

Find all GeoMx Tech Notes and White Papers in the NanoString University Document Library.

• Unlocking Predictive Potential for Response to Immunotherapy
• Cancer Transcriptome Atlas: Deep Spatial Characterization of the Colorectal Cancer Tumor Microenvironment
• Introduction to GeoMx Cancer Transcriptome Atlas: Normalization
• Spatial Profiling of COVID-19 Samples
• GeoMx DSP Operation Guide for Service Providers
• Combined Workflow of GeoMx Cancer Transcriptome Atlas and RNAscope Assays
• Multi-step Antibody Validation for the GeoMx Digital Spatial Profiler
• Multiplex, Spatially Resolved Protein Analysis (Abcam)
• Integrated and Automated Workflow for High Plex RNA and Protein Profiling (Leica)
• Selection and Validation of GeoMx® Custom Antibody Spike-ins

• GeoMx Whole Transcriptome Atlas for the Digital Spatial Profiler: Design, Performance, and Experimental Guidelines

• Unlocking Predictive Potential for Response to Immunotherapy
• Cancer Transcriptome Atlas: Deep Spatial Characterization of the Colorectal Cancer Tumor Microenvironment
• Introduction to GeoMx Cancer Transcriptome Atlas: Normalization
• Spatial Profiling of COVID-19 Samples
• GeoMx DSP Operation Guide for Service Providers
• Combined Workflow of GeoMx Cancer Transcriptome Atlas and RNAscope Assays
• Multi-step Antibody Validation for the GeoMx Digital Spatial Profiler
• Multiplex, Spatially Resolved Protein Analysis (Abcam)
• Integrated and Automated Workflow for High Plex RNA and Protein Profiling (Leica)
• GeoMx Whole Transcriptome Atlas for the Digital Spatial Profiler: Design, Performance, and Experimental Guidelines
• Morphology Marker Guidelines
• High-Resolution, High-Throughput Spatial Transcriptomics of Complex Tissues (Illumina)
• Introduction to GeoMx Normalization: Protein Assays
• Molecularly Guided Highly Multiplexed Spatial Profiling
• The Development and Performance of a GeoMx® Based Proteogenomic Workflow for the Detection of Both RNA and Protein on a Single FFPE Slide

• Morphology Marker Guidelines
• High-Resolution, High-Throughput Spatial Transcriptomics of Complex Tissues (Illumina)
• Introduction to GeoMx Normalization: Protein Assays
• Molecularly Guided Highly Multiplexed Spatial Profiling
• The Development and Performance of a GeoMx® Based Proteogenomic Workflow for the Detection of Both RNA and Protein on a Single FFPE Slide
• nCounter® Best Practices: How to Make a Synthetic Calibration Sample – Tech Note

Gene Expression

• Best Practices for Clinical Biomarker Studies Using the nCounter Platform
• Best Practices for PanCancer IO 360™ Gene Expression Panel for FFPE Samples
• Best Practices for Breast Cancer 360™ Gene Expression Panel
• nCounter Gene Expression Profiling from Low Sample Input
• Using nCounter Analysis System with FFPE Samples for Gene Expression Analysis
• New Insights into Neuroscience Research
• Neuropathology Application Note
• Using the nCounter® Analysis System with FFPE Samples for Gene Expression Analysis
• Multiplexed Cancer Immune Response Analysis nCounter® PanCancer Immune Profiling Panel for Gene Expression
• Reference Genes for Normalization of Expression Data
• Panel Standards

Gene Fusions

• Optimal Thaw/Freeze Cycle Analysis for nCounter® Lung Gene Fusion Panel

miRNA and CNV

• Tech Note for nCounter miRNA Expression Analysis in Plasma and Serum Samples
• Targeted miRNA Discovery and Validation Using the nCounter® Platform
• Applications with Exosomes and Extracellular Vesicles in miRNA Research
• Analyzing FFPE Specimens with the nCounter CNV Assay

3D Biology

• Ultra-Low Sample Inputs for RNA:Protein Profiling Experiments with nCounter® Vantage 3D™ Universal Cell Capture Kits
• NanoString Profiling in Immuno-Oncology
• Uncovering the Tumor-Immune System Interaction Using the nCounterVantage™ RNA:Protein Immune Cell Panels
• PBMC Tips and Tricks
• nCounter Vantage 3D Protein Assay Normalization and Data Analysis

Product Bulletins and Data Sheets

nCounter

• nCounter Analysis System
• nCounter PlexSet™ Reagents
• nCounter miRNA Expression Panels
• nCounter miRGE Assay

nCounter – Oncology

• Vantage 3D™ RNA Protein Solid Tumor Assay
• Vantage 3D™ RNA Protein Heme Assay
• v2 Cancer CNV Assay
• Tumor Signaling 360 Panel
• PanCancer IO 360™ Panel
• Myeloid Innate Immunity Panel
• Metabolic Pathways Panel
• Low RNA Input Kit
• Hallmarks of Cancer
• CAR-T Characterization Panel
• Copy Number Variation Assay
• Breast Cancer 360™ Panel

nCounter – Neuroscience

• Neuroinflammation Panel
• Neuropathology Panel
• Mouse and Human AD Panels
• Glial Profiling Panel
• Low RNA Input Kit

nCounter – Immunology

• Human Inflammation Panel
• Mouse Inflammation Panel
• Human Organ Transplant Panel
• Myeloid Innate Immunity Panel
• Metabolic Pathways Panel
• Low RNA Input Kit
• CAR-T Characterization Panel
• Fibrosis Panel
• Autoimmune Discovery Panel
• Autoimmune Profiling Panel
• nCounter TCR Diversity Panel-Product Bulletin-日本語カタログ

GeoMx Digital Spatial Profiler

• GeoMx RNA for DSP
• GeoMx Cancer Transcriptome Atlas
• GeoMx Whole Transcriptome Atlas
• GeoMx Neuroscience Protein
• DSP IO Protein
• GeoMx Cancer Transcriptome Atlas-日本語カタログ

Product Specifications

• nCounter Human v2 miRNA Expression Assay
• nCounter miRGE Assay
• nCounter XT Plex2 Assay

Panel Gene Lists

• nCounter Autoimmune Discovery Consortium
• nCounter Autoimmune Profiling (Human)
• nCounter Autoimmune Profiling (Mouse)
• nCounter Breast Cancer 360 Panel
• nCounter CAR-T Characterization Panel
• nCounter Customer Assay Evaluation (CAE)
• nCounter Human AD Panel
• nCounter Human Immunology v2
• nCounter Human Inflammation v2
• nCounter Human Kinase v2
• nCounter Human v3 miRNA
• nCounter Human Neuroinflammation
• nCounter Human Neuropathology
• nCounter Immune Exhaustion Panel (Human)
• nCounter Immune Exhaustion Panel (Mouse)
• nCounter Mouse AD Panel
• nCounter Mouse Immunology
• nCounter Mouse Inflammation v2
• nCounter Mouse v1.5 miRNA
• nCounter Mouse Neuroinflammation
• nCounter Mouse Neuropathology
• nCounter NHP Immunology Consortium
• Metabolic Pathways Panel Gene List (Human)
• Metabolic Pathways Panel Gene List (Mouse)
• nCounter PanCancer Human Immune Profiling
• nCounter PanCancer Human Pathways
• nCounter PanCancer Human Progression
• nCounter PanCancer Human IO 360 Panel
• nCounter PanCancer Mouse Immune Profiling
• nCounter PanCancer Mouse Pathways
• nCounter PanCancer Mouse IO 360
• nCounter Rat miRNA Expression Assay
• nCounter Vantage Leukemia Gene Fusion Panel
• nCounter Vantage Lung Gene Fusion Panel
• nCounter Vantage Protein Immune Cell Profiling Panel (D)
• nCounter Vantage Protein Immune Cell Profiling Panel (R)
• nCounter Vantage RNA Adaptive Immunity Panel
• nCounter Vantage RNA Cancer Metabolism Panel
• nCounter Vantage RNA Cellular Profiling Panel
• nCounter Vantage RNA Innate Immunity Panel
• nCounter Vantage RNA Intracellular Signaling Panel
• nCounter Vantage RNA Panel for DNA Damage and Repair
• nCounter Vantage RNA-Protein Immune Cell Profiling Panel
• nCounter Vantage RNA-Protein Solid Tumor Signaling Pathways Panel
• nCounter Vantage RNA-Protein Solid Tumor Signaling Pathways Panel for FFPE
• nCounter Vantage RNA Wnt Pathways Panel

Safety Information (SDS)

CodeSet & TagSet

• Reporter CodeSet, Capture ProbeSet, Elements TagSet and PlexSet (English) (LBL-10597)
  • Reporter CodeSet, Capture ProbeSet, Elements TagSet and PlexSet (French) (LBL-10598)
  • Reporter CodeSet, Capture ProbeSet, Elements TagSet and PlexSet (Spanish) (LBL-10599)
  • Reporter CodeSet, Capture ProbeSet, Elements TagSet and PlexSet (German) (LBL-10600)
  • Reporter CodeSet, Capture ProbeSet, Elements TagSet and PlexSet (Danish) (LBL-10793)

Master Kit Components

• Cartridges (English) (LBL-10470)
  • Cartridges (Spanish) (LBL-10474)
  • Cartridges (French) (LBL-10473)
  • Cartridges (German) (LBL-10674)
  • Cartridges (Chinese) (LBL-10472)
  • Cartridges (Danish) (LBL-10796)
• Hybridization Buffer (English) (LBL-10603)
  • Hybridization Buffer (French) (LBL-10604)
  • Hybridization Buffer (Spanish) (LBL-10605)
  • Hybridization Buffer (German) (LBL-10584)
  • Hybridization Buffer (Danish) (LBL-10794)
• Prep Plate (English) (LBL-C0010)
  • Prep Plate (French) (LBL-C0014)
  • Prep Plate (Spanish) (LBL-C0162)
  • Prep Plate (German) (LBL-10585)
  • Prep Plate (Danish) (LBL-10795)

SPRINT Reagent Pack

• Reagent A (US-English) (LBL-10757)
  • Reagent A (French) (LBL-10758)
  • Reagent A (Spanish) (LBL-10759)
  • Reagent A (German) (LBL-10760)
  • Reagent A (UN-English) (LBL-10761)
• Reagent B (US-English) (LBL-10762)
  • Reagent B (French) (LBL-10763)
  • Reagent B (Spanish) (LBL-10764)
  • Reagent B (German) (LBL-10765)
  • Reagent B (UN-English) (LBL-10766)
• Reagent C (English) (LBL-10257)
  • Reagent C (French) (LBL-10681)
  • Reagent C (Spanish) (LBL-10682)
  • Reagent C (German) (LBL-10683)
• Hybridization Buffer (LBL-10603)

GeoMx™ Digital Spatial Profiler

• Nucleic Acid Stain (English) (LBL-10646)
  • ​Nucleic Acid Stain (French) (LBL-10647)
  • Nucleic Acid Stain (Spanish) (LBL-10648)
  • Nucleic Acid Stain (German) (LBL-10649)
• Visualization Marker (English) (LBL-10642)
  Includes: anti-CD45 Visualization Marker; anti-PanCK Visualization Marker; anti-S100B/a-PMEL
  • Visualization Marker (French) (LBL-10643)
  • Visualization Marker (Spanish) (LBL-10644)
  • Visualization Marker (German) (LBL-10645)
• Fluidic Introduction Solution (US English) (LBL-10797)
  • Fluidic Introduction Solution (UN GHS) (LBL-10798)
  • Fluidic Introduction Solution (French) (LBL-10799)
  • Fluidic Introduction Solution (German) (LBL-10800)
  • Fluidic Introduction Solution (Spanish) (LBL-10801)

Protein Labeling Kit

• Activated Oligos (US English) (LBL-10886-01)
• Activated Oligos (UN English) (LBL-10887-01)
• Activated Oligos (French) (LBL-10888-01)
• Activated Oligos (German) (LBL-10889-01)
• Activated Oligos (Spanish) (LBL-10890-01)
• Buffer A (US English) (LBL-10891-01)
• Buffer A (UN English) (LBL-10892-01)
• Buffer A (French) (LBL-10893-01)
• Buffer A (German) (LBL-10894-01)
• Buffer A (Spanish) (LBL-10895-01)
• Buffer C (US English) (LBL-10896-01)
• Buffer C (UN English) (LBL-10897-01)
• Buffer C (French) (LBL-10898-01)
• Buffer C (German) (LBL-10899-01)
• Buffer C (Spanish) (LBL-10900-01)

miRNA Reagents

• miRNA Annealing Buffer (English) (LBL-C0049)
  • miRNA Annealing Buffer (French) (LBL-C0154)
  • miRNA Annealing Buffer (Spanish) (LBL-C0155)
• Polyethylene Glycol (English) (LBL-C0052)
  • Polyethylene Glycol (French) (LBL-C0164)
  • Polyethylene Glycol (Spanish) (LBL-C0165)
  • Polyethylene Glycol (German) (LBL-10669)

Additional Reagents

• Probe Pools, Target Pools, Panel Standards and Assay Controls (English) (LBL-10601)
  Includes: miRNA Assay Controls; Custom CNV Fragmentation Controls; miRNA Tag Reagent; Oligo Pools; Panel Standards; PCR Primers; Primer Pools; Probe Pools; Target Pools
  • Probe Pools, Target Pools, Panel Standards and Assay Controls (French) (LBL-10586)
  • Probe Pools, Target Pools, Panel Standards and Assay Controls (Spanish) (LBL-10587)
  • Probe Pools, Target Pools, Panel Standards and Assay Controls (German) (LBL-10588)
• RNA Reference Samples and Training Samples (English) (LBL-10589)
  Includes: Anti-PD1 Controls; Anti-PD1 Reference Samples; Clinical Controls; LST Reference Samples; LST Training Samples; Prosigna Reference Samples; Prosigna® Training Samples
  • RNA Reference Samples and Training Samples (French) (LBL-10590)
  • RNA Reference Samples and Training Samples (Spanish) (LBL-10591)
  • RNA Reference Samples and Training Samples (German) (LBL-10592)
• Antibody Mix (English) (LBL-10123)
  Includes: Antibody Conjugate Mix; Antibody Mix; RNA:Protein; Protein Kit
  • Antibody Mix (French) (LBL-10639)
  • Antibody Mix (Spanish) (LBL-10640)
  • Antibody Mix (German) (LBL-10641)
• Custom Multiplex PCR 5x Master Mix (English) (LBL-10457)
  Includes: 5x dU Amp Master Mix (SNV)
  • Custom Multiplex PCR 5x Master Mix (French) (LBL-10481)
  • Custom Multiplex PCR 5x Master Mix (Spanish) (LBL-10477)
• Multiplex PCR 5x Master Mix (English) (LBL-10459)
  Includes: 5x dT Amp Master Mix (Low Input)
  • Multiplex PCR 5x Master Mix (French) (LBL-10483)
  • Multiplex PCR 5x Master Mix (Spanish) (LBL-10479)
• Custom Protoscript II 10X Reaction Primer Mix (English) (LBL-10735)
  Includes: 10x RT Primer Mix (Low Input)
  • Custom Protoscript II 10X Reaction Primer Mix (French) (LBL-10736)
  • Custom Protoscript II 10X Reaction Primer Mix (Spanish) (LBL-10737)
• Buffer H – with ProClin 950 (US-English) (LBL-10767)
  • Buffer H – with ProClin 950 (French) (LBL-10768)
  • Buffer H – with ProClin 950 (Spanish) (LBL-10769)
  • Buffer H – with ProClin 950 (German) (LBL-10770)
  • Buffer H – with ProClin 950 (UN-English) (LBL-10771)
• Buffer H – with Sodium azide (US-English) (LBL-10663)
  • Buffer H – with Sodium azide (French) (LBL-10653)
  • Buffer H – with Sodium azide (Spanish) (LBL-10654)
  • Buffer H – with Sodium azide (German) (LBL-10655)
• Buffer LH (English) (LBL-10374)
  • Buffer LH (French) (LBL-10675)
  • Buffer LH (Spanish) (LBL-10676)
• Buffer FC (English) (LBL-10670)
• Buffer FD (English) (LBL-10437)
  • Buffer FD (French) (LBL-10677)
  • Buffer FD (Spanish) (LBL-10678)
• Buffer PW (English) (LBL-10438)
  • Buffer PW (French) (LBL-10679)
  • Buffer PW (Spanish) (LBL-10680)
• Buffer S – with ProClin 950 (US-English) (LBL-10772)
  • Buffer S – with ProClin 950 (French) (LBL-10773)
  • Buffer S – with ProClin 950 (Spanish) (LBL-10774)
  • Buffer S – with ProClin 950 (German) (LBL-10775)
  • Buffer S – with ProClin 950 (UN-English) (LBL-10776)
• Buffer S – with Sodium azide (US-English) (LBL-10662)
  • Buffer S – with Sodium azide (French) (LBL-10650)
  • Buffer S – with Sodium azide (Spanish) (LBL-10651)
  • Buffer S – with Sodium azide (German) (LBL-10652)
• Buffer T (US-English) (LBL-10593)
  • Buffer T (French) (LBL-10594)
  • Buffer T (Spanish) (LBL-10595)
  • Buffer T (German) (LBL-10596)
  • Buffer T (UN-English) (LBL-10777)
• miRNA Annealing Buffer (US-English) (LBL-C0049)
  • miRNA Annealing Buffer (French) (LBL-C0154)
  • miRNA Annealing Buffer (Spanish) (LBL-C0155)
  • miRNA Annealing Buffer (German) (LBL-10753)
  • miRNA Annealing Buffer (UN-English) (LBL-10778)
• Enzymes and Enzyme Mixtures (English) (LBL-10684)
  • Enzymes and Enzyme Mixtures (French) (LBL-10685)
  • Enzymes and Enzyme Mixtures (Spanish) (LBL-10686)
  • Enzymes and Enzyme Mixtures (German) (LBL-10687)
• Cell Capture Beads and Reagents (English) (LBL-10688)
  • Cell Capture Beads and Reagents (French) (LBL-10689)
  • Cell Capture Beads and Reagents (Spanish) (LBL-10690)
  • Cell Capture Beads and Reagents (German) (LBL-10691)