CosMx™ SMI for Spatial Single-Cell Imaging
True Single-Cell In Situ Solution
Introducing the CosMx™ Spatial Molecular Imager
Elevate your single-cell research with CosMx, a Spatial Multiomics Single-Cell Imaging Platform.
CosMx SMI is the first high-plex in situ analysis platform to provide spatial multiomics with formalin-fixed paraffin-embedded (FFPE) and fresh frozen (FF) tissue samples at cellular and subcellular resolution. CosMx SMI enables rapid quantification and visualization of up to 1,000 RNA and 100 validated protein analytes. It is the flexible, spatial single-cell imaging platform that will drive deeper insights for cell atlasing, tissue phenotyping, cell-cell interactions, cellular processes, and biomarker discovery.
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Comprehensive answers with 1000-plex RNA assays in a single experiment
Uncover biological insights with predefined high-plex panels encompassing genes for cell typing, cell states, cell-cell interaction and much more. Get the most out of every experiment with our cost effective predefined 1000-plex panel with the option to customize.
Get single-cell gene expression in challenging FFPE tissues with spatial context.
Melanoma FFPE tissue probed with 1000-plex RNA panel to detect spatial localization of transcripts in intact tissue.
Detect low expressing genes with highly sensitive CosMx SMI
| Genes | Expression level | Copies per cell (Max.) |
|---|---|---|
| MALAT1 | High | 256 |
| FOS | Medium | 40 |
| LY6D | Low | 24 |
Total number of cells analyzed = 800,327
Total transcripts detected = 259,604,214
Number of transcripts / cell (Max) = 2000
Number of transcripts / cell (Mean) = 257
Shown here are the transcripts detected per cell in non-small cell lung cancer (NSCLC) FFPE tissue probed with a 1000-plex gene panel (left) and total transcript distribution in the NSCLC tissue (right). CosMx SMI offers high sensitivity and high dynamic range to capture low copy number gene transcripts at the single cell level.
Multi-modal approach for true single-cell segmentation
Multi-modal cell segmentation process provides accurate cell boundaries detection. CosMx cell segmentation uses cell membrane and morphology marker protein images, machine-learning augmented cell segmentation algorithm and transcript-based segmentation refinement to achieve precise single-cell segmentation in morphologically intact tissue.
Detect up to 1000-plex RNA and 100-plex protein in intact tissue samples
In addition to RNA, detect and spatially visualize up to 100 proteins on the intact tissues. Shown here are 7 protein targets visualized on a normal colon FFPE tissue.
How it works
CosMx SMI is an integrated system with mature cyclic fluorescent in situ hybridization (FISH) chemistry, high-resolution imaging readout, interactive data analysis and visualization software.
Easy Sample Preparation, Compatible with Any Sample Type
Automated Cyclic in situ Hybridization Chemistry
Applications
CosMx Spatial Molecular Imager is the most flexible and robust spatial single-cell imaging platform for:
- Cell Atlas and Characterization: Define cell types, cell states, tissue microenvironment phenotypes, and gene expression networks within spatial context.
- Cell-cell Interaction: Understand biological process controlled by ligand-receptor interactions.
- Spatial Biomarkers: Quantify change in gene expression based on treatment and identify single-cell subcellular biomarkers with spatial context.
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Related Resources
Technical Publication
High-Plex Multiomic Analysis in FFPE Tissue at Single-Cellular and Subcellular Resolution by Spatial Molecular Imaging
Grant Support Package
