ACD Partnership
The power of single-molecule RNAscope™ visualization combined with high-plex GeoMx® RNA assays.
- Molecularly guide high-plex spatial analyses on GeoMx DSP with single cell resolution
- Validated protocol with qualified RNAscope probes
- Integrated manual and automated workflows
- Fresh frozen and formalin-fixed paraffin-embedded sample compatible
Technology Overview
- Visualize mRNA transcript for cytokines, transcription factors, and low expressing targets
- Choose regions of interest on GeoMx DSP based on critical gene expression for high-plex profiling
- Quantify 1,800+ gene targets in each region of interest with the GeoMx Cancer Transcriptome Atlas
Qualified RNAscope Probe Combinations
- Target interesting immune biology in fresh frozen (FF) and formalin-fixed paraffin embedded (FFPE) tissues
- Choose a probe combination from our list or design your own combination
- Review GeoMx Manual that integrates RNAscope into the GeoMx workflow
- To learn more or order RNAscope probes listed below visit ACD
Qualified RNAscope Probe Combinations
| TARGET BIOLOGY | PROBE 1 | PROBE 2 | PROBE 3 |
|---|---|---|---|
| Immune checkpoints | PDCD1 (PD-1) | CD274 (PD-L1) | CD3E |
| Tumor vs. Stroma | PanCK | PTPRC (CD45) | CD3E |
| Cytotoxic T Lymphocytes (Set 1) | CD8A | GZMB | CD3E |
| Cytotoxic T Lymphocytes (Set 2) | CD8A | IFNG | |
| T regs | CD4 | FOXP3 | |
| Activated T regs | CD4 | FOXP3 | TIGIT |
| Immune suppression | TGFB1 | FOXP3 | |
| Macrophages | CD68 | CD163 | CD3E |
| M1 Macrophages | CD68 | CXCL10 | |
| M2 Macrophages | CD163 | CCL22 | CD3E |
For Research Use Only. Not for use in diagnostic procedures.