FFPE Simplified

Helping Your Research

FFPE tissue samples (also known as Formalin-Fixed Paraffin-Embedded samples) are notoriously difficult for molecular analysis with high variability, low yield, and in many cases, high degradation.

The nCounter® Analysis System eliminates these challenges by utilizing molecular “barcodes” and single molecule imaging to directly hybridize and detect hundreds of unique transcripts in a single reaction without any amplification steps that might introduce bias. With a single curl of FFPE tissue, nCounter generates data correlating with that generated from matched fresh frozen material.

Performance Data

Independent research by Reis PP, et al., “mRNA Transcript Quantification in Archival Samples Using Multiplexed, Color-coded Probes,” evaluates the results of FFPE tissue analysis performed on the nCounter System compared to qPCR. The figure on the left shows nCounter data which has a correlation coefficient between FFPE and fresh-frozen of 0.90, and the figure on the right shows equivalent data for qPCR with a correlation coefficient of 0.50. This illustrates the superiority of nCounter data to that of qPCR for gene expression analysis of FFPE tissue samples.

nCounter and qPCR using FFPE tissue samples

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