How to Perform the miRNA Expression Assay

This course will show you how to prepare your samples and set up the hybridization reactions for the nCounter miRNA Expression Assay. We will go over:

  • NanoString-supplied Reagents
  • Proper Sample Handling Techniques
  • miRNA Sample Preparation
    • Annealing Protocol
    • Ligation Protocol – Pay close attention to this step!
    • Purification Protocol
  • miRNA Assay Protocol
    • Hybridization reaction setup

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