Live from the Amsterdam Customer Experience lab: focus on single-cell spatial with CosMx SMI

Single cell spatial proteomics of HER2-positive ductal carcinoma in situ and invasive breast cancer

AVAILABLE ON DEMAND

As part of our webinar series dedicated to Women’s Health Awareness Month, this ‘Live from the Cx lab’ episode will feature the work done by Helga Bergholtz from Oslo University Hospital on ‘Single cell spatial proteomics of HER2-positive ductal carcinoma in situ and invasive breast cancer’.

Our Sr FAS Amanda Mason will introduce the technology behind the CosMx Spatial Molecular Imager and show the instrument installed in the lab.

More than half of ductal carcinoma in situ (DCIS) cases never become invasive. The molecular phenotype of cancer cells in DCIS and invasive ductal carcinoma (IDC) is surprisingly similar, pointing to the microenvironment as an important regulator of invasion. The aim of Helga’s study was to compare the immune microenvironment in HER2-positive DCIS and IDC. We performed single cell spatial proteomic analyses of one DCIS and one IDC using CosMx SMI platform and the Immuno-oncology protein panel. They found heterogeneity in tumor growth pattern, immune cell infiltration and protein expression within tumors. In particular, there was higher abundance of B cells in DCIS, while CD8+ T cells were more abundant in IDC. Although this study includes only two cases, it highlights B cells as important players in the immune landscape of breast tumor progression and provides a unique possibility to explore more in-depth the heterogeneity of immune cell infiltration on a single-cell level.