GeoMx® Neuroscience Protein Panels

For nCounter Readout

Unravel the complexity of the CNS

Profile up to 96 human or mouse proteins relevant to neuroscience research from distinct tissue compartments and cell type populations with the modular design of the GeoMx Neuroscience Protein Panels. Take advantage of targeted content and expedited readout with the nCounter® Analysis System and discover novel biomarkers of disease and unique drug targets. With no specialized tissue mounting required and a workflow compatible with standard histology staining, you can easily add spatial proteomics to your lab’s capabilities.

Circular graphic for GeoMx Neuroscience Protein Panels
Illustration of modular panel design with protein core surrounded by modules

The Neural Cell Profiling Core contains 20 targets designed for broad cell profiling and includes necessary controls for all GeoMx DSP experiments. Up to 6 modules plus 10 custom targets can be added to select the content most relevant to your research. All cores and modules contain validated antibodies conjugated to unique oligonucleotide barcodes via a UV photocleavable linker. After region of interest (ROI) selection and UV cleavage of the oligonucleotides, each barcode is recognized by a unique reporter probe that contains a fluorescent barcode. Reporter probes are imaged and counted by the nCounter® Analysis System to provide a direct, digital readout of protein expression that is mapped back to each corresponding ROI. 

How It Works

Built with a modular design that allows you to mix and match content, the GeoMx Neuroscience Protein Panels allow you to profile the in situ spatial expression of up to 96 human or mouse proteins involved in neural cell biology and neurodegenerative disease pathology.

01:

Curated content designed for neurodegenerative and neuroimmunological research

02:

Modular design allows selection of up to six 10-plex modules to add to the Neural Cell Profiling Core

03:

Validated in multiplex format for use in human and mouse FFPE or fresh frozen tissue

04:

Customizable with up to 5 additional targets of interest with the DIY Protein Barcoding Kit or up to 10 antibodies with the Protein Barcoding Service

05:

For use with nCounter readout and compatible with DSP Data Analysis Software

Curated Content for Neuroscience

All GeoMx Protein Assays undergo extensive validation to ensure high quality GeoMx DSP data.

5-step diagram: Ordering Screen -> Post-Conjugation Screen -> Sensitivity/Specificity Screen -> Interaction Screen -> TMA Screen

Each antibody is tested for specificity before and after oligonucleotide conjugation to ensure performance in the assay (Figure 1). Additionally, spike-in of each module does not affect antibody specificity, demonstrating robust multiplexing capability (Figure 2).

DAB - Brain NeuN and Iba1 images


Figure 1: Example NeuN (DPROT_00101.1) and Iba1 (DPROT_00098.1) are tested for specific staining pre- and post- conjugation to a specific indexing oligonucleotide to ensure conjugation does not alter specificity.
Three graphs demonstrating lack of antibody-antibody interference

Figure 2: Example signal for the Neuro Cell Profiling Core is compared to the Neuro Cell Profiling Core plus individual Modules to ensure no antibody-antibody interference. Single antibody interference testing is also performed prior to core + module testing.

Related Resources

Brochure/eBook GeoMx DSP – Brochure
Product Bulletin GeoMx Protein Barcoding Kits – Product Bulletin
Whitepaper Multistep Antibody Validation for the GeoMx – Whitepaper
App Note/Tech Note Introduction to GeoMx Normalization: Protein
Manual/Instructions GeoMx® Data Analysis for nCounter Readout

Request a Quote

Contact our helpful experts and we’ll be in touch soon.