nCounter® CodeSet Plus

• Expand a new CodeSet: Add up to 55 of your favorite genes or a collection of specific controls to any new CodeSet purchase
• Update an existing CodeSet: Utilize results from your experiments and add new genes as you update your previously purchased CodeSet
• Create specific control sets: Create multiple Plus control sets for use with different sample or cell types
• Add more genes later: Finalize your initial gene list more quickly with the ability to add additional genes at a later time. 

Performance of Panel Plus Gene Sets with pre-designed Panels (Standard Gene Expression and Single Cell Analysis)

Two experiments were performed by adding Panel Plus genes to the Cancer Reference Panel. Data in each figure below demonstrate that addition of CodeSet Plus probes does not impact performance of original nCounter Panel probes or Single Cell Panel probes.

Cancer Reference Panel with 30-gene Plus
Human Reference Total RNA (100 ng) was hybridized with either nCounter Cancer Reference Panel (Original CodeSet) alone or nCounter Cancer Reference Panel with a 30-gene Panel Plus (Original CodeSet with Plus). Assays were performed in triplicate for each condition tested and normalized to internal positive controls. Average count values for each triplicate measurement were determined and plotted for all nCounter Cancer Reference Panel probes in the Original CodeSet alone (y-axis) and Original CodeSet with Plus (x-axis).

Cancer Reference Panel with 30-gene Plus

Human Reference Total RNA (100pg) was converted to cDNA and enriched via a Multiplexed Target Enrichment (MTE) with primers appropriate for downstream hybridization according to the nCounter Single Cell Expression Assay Protocol for Total RNA. The enriched cDNA was hybridized with either nCounter Cancer Reference Panel CodeSet (Original CodeSet) alone or nCounter Cancer Reference Panel with a 30-gene Panel Plus (Original CodeSet with Plus). Assays were performed in triplicate for each condition tested and normalized to internal positive controls. Average count values for each triplicate measurement were determined and plotted for all nCounter Cancer Reference Panel probes in the original CodeSet alone (y-axis) and Original CodeSet with Plus (x-axis).