CosMx™ SMI vs. Xenium: Superior In Situ Single-Cell Performance Study
Exciting results from the comparative study of 16 colon tissue blocks with CosMx SMI & Xenium using data from Parambir Dulai, MD from Northwestern Medicine Feinberg School of Medicine in Chicago.
The CosMx™ SMI and decoder probes are not offered and/or delivered to the Federal Republic of Germany for use in the Federal Republic of Germany for the detection of cellular RNA, messenger RNA, microRNA, ribosomal RNA and any combinations thereof in a method used in fluorescence in situ hybridization for detecting a plurality of analytes in a sample without the consent of the President and Fellows of Harvard College (Harvard Corporation) as owner of the German part of EP 2 794 928 B1. The use for the detection of cellular RNA, messenger RNA, microRNA, ribosomal RNA and any combinations thereof is prohibited without the consent of the President and Fellows of Harvard College (Harvard Corporation).
Are you ready to witness a systematic comparative study for single-cell spatial analysis platforms? To assist researchers in their assessment of spatial molecular imager performance, researchers have conducted a well-controlled comparison between CosMx SMI and Xenium.
Watch the video to see how CosMx SMI outperforms Xenium when it comes to key performance and discoverable biology:
Key takeaways from Dr. Dulai’s webinar:
- CosMx SMI provides higher genomic breadth
- CosMx SMI results in higher cell typing consistency
- Cell segmentation is important for data accuracy
- CosMx SMI performs consistently across samples independent of RNA quality
Comparison of CosMx 1K Universal & Xenium 377-plex Multi-Tissue and Cancer Panel
CosMx measures ~6x more genes per cell than Xenium from 16 colon tissue samples
Comparison attempts to date have failed to consider critical experimental design considerations
Importance for controlled variables:
- Consistent sample preparation time is crucial
- Avoid biases caused by deviating from recommended protocols
Necessity for multiple biological replicates:
- Enhances reliability and generalizability of findings
- Reduces risk of inaccurate conclusions based on limited data
| Previous Studies | This Study | |
|---|---|---|
| Just one biological replicate (n=1) | ≠ | 16 tissue blocks from 11 patients |
| 44-day old TMA sections | Fresh-cut samples |