NanoString Blog

Spatial Transcriptomics

What is transcriptomics?

The transcriptome is the sum of all the RNA in an organism’s cells and tissues being actively transcribed at any given time from genomic DNA. It includes protein-coding messenger RNA (mRNA)…

Nirupama Deshpande / July 5, 2023

Digital Spatial Profiling

What is the principle of immunofluorescence?

Immunofluorescence staining was first used in 1942 and has since remained a highly reliable and powerful technique for a wide range of research and diagnostics. The principle of immunofluorescence is…

Nirupama Deshpande / July 4, 2023


What does nTPM mean in the protein atlas?

The goal of the Human Protein Atlas (HPA) project is to map all human proteins in cells, tissues, and organs.  Further, it aims to annotate the function and spatial distribution of…

Nirupama Deshpande / July 3, 2023
Geo location

Digital Spatial Profiling GeoMx DSP

What is a morphology marker?

Understanding tissue morphology is crucial to identify and separate regions of tissue into meaningful biological groupings for image analysis of tissue sections. This is done using morphological markers, most of…

Nirupama Deshpande / July 2, 2023


What is immunofluorescence used for?

Immunofluorescence techniques are being increasingly used as an imaging and diagnostic tool in clinical pathology, oncology, and infectious disease, particularly for the study of biomarkers related to cancer diagnosis, prognosis,…

Nirupama Deshpande / July 1, 2023

Digital Spatial Profiling

How to download data from the human protein atlas

The Human Protein Atlas (HPA) is a Swedish-based program initiated in 2003 and has become an important resource for both basic and clinical research. It is one of the largest…

Nirupama Deshpande / June 30, 2023
CosMx tissue image

Spatial Biology

What are NanoString platforms used for?

A pioneer in the field of spatial biology, NanoString Technologies has developed automated workflow platforms enabling researchers to visualize and quantify gene and protein expression in tissue sections down to…

Nirupama Deshpande / June 29, 2023


How is immunofluorescence staining done?

Immunofluorescence (IF) staining is a widely used technique that allows for the detection and localization of antigens in any given tissue or cell type. IF methods use combinations of specific…

Nirupama Deshpande / June 28, 2023

Spatial Transcriptomics

An Integrated Omics Approach: The Human Protein Atlas

Although humans have been using atlases for thousands of years, the word atlas was first coined in 1595 by the Flemish map maker Gerardus Mercator. In geographical terms, an atlas…

Nirupama Deshpande / June 27, 2023

Spatial Transcriptomics

What is the human protein atlas used for?

In order to understand the role of each protein in various biological processes such as cell organization, signaling pathways, or disease mechanisms, it becomes essential to study protein expression with…

Nirupama Deshpande / June 26, 2023
Geo location

Digital Spatial Profiling

What is digital spatial profiling?

The function of biological systems depends on the spatial organization of their cells. Over the past decade, innovative technologies have emerged that can spatially localize and quantify gene expression of…

Nirupama Deshpande / June 25, 2023


Immunofluorescence Staining: An Overview

Immunofluorescence staining is an incredibly powerful yet simple imaging technique that optically detects the localization, distribution, and abundance of proteins at a cellular and intracellular level using fluorescent tags on…

Nirupama Deshpande / June 24, 2023