GeoMx®
Morphology Markers

These morphology markers are commonly used to profile tumor and immune compartments or neuronal populations. These fluorescent dye-conjugated antibodies are validated for a diverse array of tissues and GeoMx assays across mouse and human tissues. These kits are utilized with a SYTO 13 nucleic acid stain kit. The Morphology Kit configuration files are used to auto-populate key target information on the GeoMx scan setup step and can be downloaded here.

These markers are obtained from our partners including Abcam, Biolegend, and other vendors, and tested in GeoMx assays following the “Qualified” and “Verified” approaches. Qualified marker tests showed the expected staining pattern typically in a single tissue. Verified markers had gone through more extensive testing on multiple tissues, when possible, and were used for GeoMx ROI segmentation. Moreover, target specific positive tissue staining as verified by an experienced pathologist.

The Technology Access Program (TAP) allows users to run small trial projects. Through this work, the program has developed a long list of morphology markers observed to work well with specific tissues under specific conditions. A list of successfully utilized antibodies, RNAscope probes, and the tested tissues has been compiled to help users with their research projects: TAP tested morphology markers

The NanoString user community works with a wide range of morphology markers and tissue types. We are compiling a list of pre-screened markers that worked well with specific tissues by our customers. We encourage our users to share markers that have successfully been used in a GeoMx experiment. Let the research community know by submitting your verified morphology markers via this Community Verified Morphology Markers Form

For RNA morphology targets, you can learn about our joint workflow solution with our partner Advanced Cell Diagnostics (ACD) and how to select qualified RNAscope probe combinations: ACD and NanoString Partnership

For antibody morphology markers, it is recommended to begin with those already validated for IF/IHC assays by our antibody partners, Abcam or Biolegend, or another provider. Once selected, it is important that they are tested using the specific staining protocols used for GeoMx. Please refer to Morphology Marker Guidelines for further details.​

Canopy Biosciences is a highly experienced CRO in spatial biology and single-cell analysis research. Canopy has developed a list of NanoString verified markers and validated multiplex assays. This catalog covers key immunology, oncology, and neuroscience markers and demonstrates marker validation capabilities. For more info on Canopy’s services, specifically their GeoMx services and morphology marker validation, visit www.canopybiosciences.com.